RNA Methods: RNA Extraction from Plasma

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Optimization of RNA Extraction from Rat Pancreatic Tissue

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optimization of rna extraction from rat pancreatic tissue

background: optimized rna extraction from tissues and cell lines consists of four main stages regardless of the method of extraction: 1) homogenizing, 2) effective denaturation of proteins from rna, 3) inactivation of ribonuclease, and 4) removal of any dna, protein, and carbohydrate contamination. isolation of undamaged intact rna is challenging when the related tissue contains high levels of ...

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ژورنال

عنوان ژورنال: BioTechniques

سال: 2009

ISSN: 0736-6205,1940-9818

DOI: 10.2144/000113235